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Alterations in the tumor suppressor protein p16Ink4a following co-culture of osteosarcoma cells with osteoblastic cells.
Osteosarcoma is a bone tumor which mainly affects adolescents and young adults. Its therapy remains an unresolved problem, particularly in older patients. Several proteins, including p16, involved in the induction and progression of the disease were already identified, but their biological function and inter-cellular interaction are still poorly known. To study the mechanisms regulating the growth of osteosarcoma cells, we chose the CH-297 M cell line, which is able to mimic the growth of the human osteosarcoma cell line, MG63. Both cell types secrete soluble factors that stimulate each other. We have shown that CH-297 M tumor cells stop proliferating after being co-cultured with MG-63 osteoblast-like cells. This cell-cell interaction is associated with an inhibition of the expression of the p16 cell cycle regulator as well as an increased expression of the cell cycle-related proteins, cyclin D1, cyclin E and Rb. We conclude that p16 expression could be involved in the mechanism of the cell-cell interaction observed in our co-culture model.Vacuum Packing Molds & Vacuum Makers

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